[Pombelist] (no subject)

[Dana Laor]

Dear all,

In order to detect histone modifications I have been trying to do chromatin
IP. The problem is that I always get a signal in the no-antibody control
sample. The signal is as strong as the antibody sample. For the lysis and
the washing I use lysis buffer with 0.5M NaCl. In order to get rid of the
signal I tried to pre-cleared the beads (separose beads) for an hour with no
success. The primers are not contaminated since there is no signal in the
no-DNA sample. Does anyone have any idea what I should do?

I will appreciate any suggestions.

Thanks,
Dana

-- 
Dana Laor
Prof. Martin Kupiec's research group
Dept of Molecular Microbiology and Biotechnology
Tel Aviv University
Ramat Aviv, Tel Aviv, 69978
Israel
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://publists.sanger.ac.uk/pipermail/pombelist/attachments/20101208/f0bde6a1/attachment-0001.htm>