[Pombelist] (no subject)
laor.dana at gmail.com
Wed Dec 8 11:21:06 GMT 2010
In order to detect histone modifications I have been trying to do chromatin
IP. The problem is that I always get a signal in the no-antibody control
sample. The signal is as strong as the antibody sample. For the lysis and
the washing I use lysis buffer with 0.5M NaCl. In order to get rid of the
signal I tried to pre-cleared the beads (separose beads) for an hour with no
success. The primers are not contaminated since there is no signal in the
no-DNA sample. Does anyone have any idea what I should do?
I will appreciate any suggestions.
Prof. Martin Kupiec's research group
Dept of Molecular Microbiology and Biotechnology
Tel Aviv University
Ramat Aviv, Tel Aviv, 69978
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