[Pombelist] PFGE

[Daniel Keifenheim]

Hello Miriam,

I don't think you have degradation issues.  If it was degradation  
there would be more of a smear below the band.

I have attached the parameters I use to separate different size  
fragments of DNA using the CHEF III system.  The only difference I can  
see is that I used 0.5X TAE instead of 1X TAE.  In my experience, salt  
concentration can have a big affect on how well the gel runs.

-------------- next part --------------
A non-text attachment was scrubbed...
Name: PFGE Parameters.doc
Type: application/msword
Size: 24576 bytes
Desc: not available
URL: <http://publists.sanger.ac.uk/pipermail/pombelist/attachments/20100118/7cf04587/attachment-0001.doc>
-------------- next part --------------



Hope this helps,
Dan Keifenheim
Rhind Lab