[Pombelist] counterselectable markers in S. pombe

Gerald Smith gsmith at fhcrc.org
Tue Apr 28 17:24:17 BST 2009


Has anyone used a substance other that FOA to block the growth of
wild-type cells but allow a mutant to grow?  Aminoadipate inhibits
wild-type S. cerevisiae but not lys2 mutants (homolog of S. pombe lys1, I
think).  Does this work for pombe?  Are there any other combinations that
work for pombe?

Thanks for your help.

Gerry Smith

______________________________________

Gerald R. Smith
Fred Hutchinson Cancer Research Center
1100 Fairview Avenue North, A1-162
P.O. Box 19024
Seattle, WA 98109-1024
Tel. 206-667-4438
FAX 206-667-6497

http://labs.fhcrc.org/gsmith/index.html
http://myprofile.cos.com/smithabc102






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