[Pombelist] FISH protocol
WXJ580 at bham.ac.uk
Wed Dec 5 14:22:10 GMT 2007
I am going to do FISH in S.pombe to check my RNA localization by four 50 nt specific probes, which have C6 amino-modification on dT and could be labelled by Cy3. I get the yeast FISH protocol from my collegue that works excellent in budding. I am wondering that what's the difference for the cell spheroplasting between fission yeast and budding yeast, simply in budding, they always use lyticase and perform it in ~10 to 15 minutes after fixation but in Nurse-lab protocol, they use zymolyase and novozyme with 90 min incubation.
Does somebody have the FISH protocol in pombe?
Thank you very much!
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